Progress on penicillinase

Studies on Penicillinase

On alkaline hydrolysis, penicillins are converted into penicilloic acids (1). Penicillinase appears to bring about the same transformation, in which a carboxyl group of pK 4.7 is generated (2). Foster (3) allowed penicillinase to act on penicillin in bicarbonate buffer in the Warburg respirometer and observed the evolution of COZ. Since such a reaction can be followed simply and with considerab...

New Assay for Penicillinase and Some Results on Penicillinase Induction.

Imsande, John (Western Reserve University, Cleveland, Ohio). New assay for penicillinase and some results on penicillinase induction. J. Bacteriol. 89:1322-1327. 1965.-A rapid, sensitive, and reliable assay of penicillinase activity is described. Studies conducted on the induction of penicillinase, with use of the new assay, show that actinomycin D inhibits growth and the induced synthesis of p...

In Vivo Studies on Staphylococcal Penicillinase.

Eyckmans, Luc (Baylor University Medical Center, Dallas, Tex.), and Ralph Tompsett. In vivo studies on staphylococcal penicillinase. J. Bacteriol. 90:589-593. 1965.-The amounts of staphylococcal penicillinase present in tissue of mice with well-established infections due to penicillin-resistant staphylococci were determined. The data indicate that, in the presence of such infection, penicillina...

Immunological specificity of Staphylococal penicillinase

Regulation of penicillinase synthesis: a mutation in Staphylococcus aureus unlinked to the penicillinase plasmid that reduced penicillinase inducibility.

A mutant of Staphylococcus aureus strain 655 was isolated that is restricted in penicillinase induction. Wild-type plasmids that bear penicillinase determinants could not be fully induced in this mutant, 655par-1; hence, the responsible mutation is not located on the plasmid. Mutant plasmid PI(258)penI443, which produces penicillinase constitutively in wild-type cells, was fully constitutive fo...